Rapid, Simple Enzyme Immunoassay for Gentamicin

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منابع مشابه

Rapid, reproducible enzyme immunoassay for gentamicin.

An enzyme immunoassay for gentamicin utilizing glucose-6-phosphate dehydrogenase was compared to the radioimmunoassay. The enzyme immunoassay for gentamicin was accurate, specific, and easily performed. It offers an ulternative method to assay aminoglycosides and could be used in institutions using enzyme immunoassay to assay other drugs.

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Enzyme immunoassay for gentamicin.

We describe a gentamicin assay in which a peroxidase-gentamicin conjugate competes with gentamicin for binding to a gentamicin antibody adsorbed to a polystyrene solid phase. The assay can be completed in 30 min and requires 50 microliter of diluted serum. The precision and accuracy are equivalent to that of a radioimmunoassay technique and the reagents are stable for several months.

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Potential liabilities of gentamicin homogeneous enzyme immunoassay.

We report two potential liabilities of the homogeneous enzyme immunoassay for the determination of serum gentamicin. One is a considerable loss of precision and accuracy at the ends of the calibration curve, and the other is an apparent loss of gentamicin with storage at -60 degrees C.

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Non-equilibrium enzyme immunoassay of gentamicin.

We describe a fast, accurate non-equilibrium enzyme immunoassay for serum gentamicin. With use of isokinetic conditions an assay takes a total of 12 min, including regeneration of the immunosorbent. The coefficient of correlation between the present method and conventional microbiological assays was 0.98.

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Assay of netilmicin, using enzyme immunoassay for gentamicin.

A homogenous enzyme immunoassay for the quantitative determination of netilmicin in serum was developed. The procedure utilizes a commercially available assay for gentamicin (EMIT; Syva Co., Palo Alto, Calif.). The method was adapted to a microcentrifugal analyzer, and log-logit regression analysis was performed with a computer. The results of samples assayed by this method correlate well with ...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 1979

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.16.1.40